{"id":1552,"date":"2019-05-11T13:46:26","date_gmt":"2019-05-11T18:46:26","guid":{"rendered":"http:\/\/my.vanderbilt.edu\/irishlab\/?p=1552"},"modified":"2019-05-11T13:46:26","modified_gmt":"2019-05-11T18:46:26","slug":"published-roussel-et-al-methods-in-molecular-biology-2019","status":"publish","type":"post","link":"https:\/\/www.irishlab.org\/index.php\/2019\/05\/11\/published-roussel-et-al-methods-in-molecular-biology-2019\/","title":{"rendered":"Published: Roussel et al., Methods in Molecular Biology 2019"},"content":{"rendered":"<p>Congrats to Mikael Roussel and Todd Bartkowiak on their publication of a review and protocol for polarization of human\u00a0monocytes and assessment by mass cytometry.<\/p>\n<ol>\n<li>Roussel M, Bartkowiak T, <strong><u>Irish JM<\/u><\/strong>. <a href=\"https:\/\/link.springer.com\/protocol\/10.1007\/978-1-4939-9454-0_14\">Picturing Polarized Myeloid Phagocytes and Regulatory Cells by Mass Cytometry<\/a>. <strong>Methods in Molecular Biology <\/strong>2019 1989:217-226. doi: 10.1007\/978-1-4939-9454-0_14.<\/li>\n<\/ol>\n<p>\u25ba This peer-reviewed publication provides a detailed protocol for 1) polarizing human monocytes into different subtypes and 2) high dimensional analysis of the resulting cells by mass cytometry.\u00a0 Polarized cell types include M1 (M_LPS, M_IFNg), M2 (M_IL-4, M_IL-10), and myeloid derived suppressor cells (MDSCs).\u00a0 Based on Roussel et al., <em>Journal of Leukocyte Biology<\/em> 2017.<\/p>\n<figure id=\"attachment_1556\" aria-describedby=\"caption-attachment-1556\" style=\"width: 2275px\" class=\"wp-caption alignleft\"><a href=\"https:\/\/my.vanderbilt.edu\/irishlab\/files\/2019\/05\/Roussel-et-al.-MiMB-2019.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-1556\" src=\"https:\/\/my.vanderbilt.edu\/irishlab\/files\/2019\/05\/Roussel-et-al.-MiMB-2019.jpg\" alt=\"Roussel et al., MiMB 2019\" width=\"2275\" height=\"2323\" srcset=\"https:\/\/www.irishlab.org\/wp-content\/uploads\/2019\/05\/Roussel-et-al.-MiMB-2019.jpg 2275w, https:\/\/www.irishlab.org\/wp-content\/uploads\/2019\/05\/Roussel-et-al.-MiMB-2019-294x300.jpg 294w, https:\/\/www.irishlab.org\/wp-content\/uploads\/2019\/05\/Roussel-et-al.-MiMB-2019-1003x1024.jpg 1003w, https:\/\/www.irishlab.org\/wp-content\/uploads\/2019\/05\/Roussel-et-al.-MiMB-2019-768x784.jpg 768w, https:\/\/www.irishlab.org\/wp-content\/uploads\/2019\/05\/Roussel-et-al.-MiMB-2019-1504x1536.jpg 1504w, https:\/\/www.irishlab.org\/wp-content\/uploads\/2019\/05\/Roussel-et-al.-MiMB-2019-2006x2048.jpg 2006w, https:\/\/www.irishlab.org\/wp-content\/uploads\/2019\/05\/Roussel-et-al.-MiMB-2019-1568x1601.jpg 1568w\" sizes=\"auto, (max-width: 2275px) 100vw, 2275px\" \/><\/a><figcaption id=\"caption-attachment-1556\" class=\"wp-caption-text\">Roussel et al., MiMB 2019<\/figcaption><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>Congrats to Mikael Roussel and Todd Bartkowiak on their publication of a review and protocol for polarization of human\u00a0monocytes and assessment by mass cytometry. Roussel M, Bartkowiak T, Irish JM. Picturing Polarized Myeloid Phagocytes and Regulatory Cells by Mass Cytometry. Methods in Molecular Biology 2019 1989:217-226. doi: 10.1007\/978-1-4939-9454-0_14. \u25ba This peer-reviewed publication provides a detailed [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[],"class_list":["post-1552","post","type-post","status-publish","format-standard","hentry","category-news"],"_links":{"self":[{"href":"https:\/\/www.irishlab.org\/index.php\/wp-json\/wp\/v2\/posts\/1552","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.irishlab.org\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.irishlab.org\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.irishlab.org\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.irishlab.org\/index.php\/wp-json\/wp\/v2\/comments?post=1552"}],"version-history":[{"count":0,"href":"https:\/\/www.irishlab.org\/index.php\/wp-json\/wp\/v2\/posts\/1552\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.irishlab.org\/index.php\/wp-json\/wp\/v2\/media?parent=1552"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.irishlab.org\/index.php\/wp-json\/wp\/v2\/categories?post=1552"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.irishlab.org\/index.php\/wp-json\/wp\/v2\/tags?post=1552"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}