{"id":1093,"date":"2015-01-01T17:08:25","date_gmt":"2015-01-01T22:08:25","guid":{"rendered":"http:\/\/my.vanderbilt.edu\/irishlab\/?p=1093"},"modified":"2015-01-01T17:08:25","modified_gmt":"2015-01-01T22:08:25","slug":"published-leelatian-et-al-methods-in-molecular-biology-2015","status":"publish","type":"post","link":"https:\/\/www.irishlab.org\/index.php\/2015\/01\/01\/published-leelatian-et-al-methods-in-molecular-biology-2015\/","title":{"rendered":"Published: Leelatian et al., Methods in Molecular Biology 2015"},"content":{"rendered":"<p style=\"text-align: justify\">Congratulations to <a href=\"http:\/\/my.vanderbilt.edu\/irishlab\/members\/current-members\/860-2\/\">Nalin Leelatian<\/a> and <a href=\"http:\/\/my.vanderbilt.edu\/irishlab\/members\/current-members\/kirsten-e-diggins-ph-d\/\">Kirsten Diggins<\/a> on this publication!<\/p>\n<p style=\"text-align: justify\">Leelatian N, Diggins KE, Irish JM. <a href=\"https:\/\/scholar.google.com\/citations?view_op=view_citation&amp;hl=en&amp;user=-IJndUMAAAAJ&amp;cstart=20&amp;pagesize=80&amp;sortby=pubdate&amp;citation_for_view=-IJndUMAAAAJ:6pF0wJmtdfAC\">Characterizing phenotypes and signaling networks of human cells by mass cytometry<\/a>. <strong>Methods in Molecular Biology<\/strong>\u00a02015.\u00a0<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC4656023\/\">PMC4656023<\/a>. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26542718\">Pubmed<\/a>. <a href=\"https:\/\/doi.org\/10.1007\/978-1-4939-2987-0_8\">DOI<\/a>.<\/p>\n<p style=\"text-align: justify\">\u25ba This peer-reviewed protocol includes isolating peripheral blood mononuclear cells (PBMC), cryopreserving live PMBC, staining cells for surface mass cytometry, and measuring signaling by phospho-specific mass cytometry (phospho-flow).\u00a0 All of the examples are human immune cells.<\/p>\n<figure id=\"attachment_420\" aria-describedby=\"caption-attachment-420\" style=\"width: 1198px\" class=\"wp-caption alignleft\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-420\" src=\"http:\/\/localhost\/irishv01\/wp-content\/uploads\/2014\/10\/Leelatian-et-al.-2015-Methods-Mol-Bio-Figure-1.jpg\" alt=\"Leelatian et al., Methods Molecular Biology 2015\" width=\"1198\" height=\"926\" srcset=\"https:\/\/www.irishlab.org\/wp-content\/uploads\/2014\/10\/Leelatian-et-al.-2015-Methods-Mol-Bio-Figure-1.jpg 1198w, https:\/\/www.irishlab.org\/wp-content\/uploads\/2014\/10\/Leelatian-et-al.-2015-Methods-Mol-Bio-Figure-1-300x232.jpg 300w, https:\/\/www.irishlab.org\/wp-content\/uploads\/2014\/10\/Leelatian-et-al.-2015-Methods-Mol-Bio-Figure-1-1024x792.jpg 1024w, https:\/\/www.irishlab.org\/wp-content\/uploads\/2014\/10\/Leelatian-et-al.-2015-Methods-Mol-Bio-Figure-1-768x594.jpg 768w\" sizes=\"auto, (max-width: 1198px) 100vw, 1198px\" \/><figcaption id=\"caption-attachment-420\" class=\"wp-caption-text\">Leelatian et al., Methods Molecular Biology 2015<\/figcaption><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>Congratulations to Nalin Leelatian and Kirsten Diggins on this publication! Leelatian N, Diggins KE, Irish JM. Characterizing phenotypes and signaling networks of human cells by mass cytometry. Methods in Molecular Biology\u00a02015.\u00a0PMC4656023. Pubmed. DOI. \u25ba This peer-reviewed protocol includes isolating peripheral blood mononuclear cells (PBMC), cryopreserving live PMBC, staining cells for surface mass cytometry, and measuring [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[],"class_list":["post-1093","post","type-post","status-publish","format-standard","hentry","category-news"],"_links":{"self":[{"href":"https:\/\/www.irishlab.org\/index.php\/wp-json\/wp\/v2\/posts\/1093","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.irishlab.org\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.irishlab.org\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.irishlab.org\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.irishlab.org\/index.php\/wp-json\/wp\/v2\/comments?post=1093"}],"version-history":[{"count":0,"href":"https:\/\/www.irishlab.org\/index.php\/wp-json\/wp\/v2\/posts\/1093\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.irishlab.org\/index.php\/wp-json\/wp\/v2\/media?parent=1093"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.irishlab.org\/index.php\/wp-json\/wp\/v2\/categories?post=1093"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.irishlab.org\/index.php\/wp-json\/wp\/v2\/tags?post=1093"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}