{"id":1026,"date":"2017-04-03T15:43:37","date_gmt":"2017-04-03T20:43:37","guid":{"rendered":"http:\/\/my.vanderbilt.edu\/irishlab\/?p=1026"},"modified":"2017-04-03T15:43:37","modified_gmt":"2017-04-03T20:43:37","slug":"leelatian-current-protocols-2017","status":"publish","type":"post","link":"https:\/\/www.irishlab.org\/index.php\/2017\/04\/03\/leelatian-current-protocols-2017\/","title":{"rendered":"Published: Leelatian et al., Current Protocols 2017"},"content":{"rendered":"<p style=\"text-align: justify\">Congratulations to <a href=\"http:\/\/my.vanderbilt.edu\/irishlab\/members\/current-members\/860-2\/\">Nalin Leelatian<\/a> and co-authors on this publication!<\/p>\n<p style=\"text-align: justify\">Leelatian N, Doxie DB, Greenplate AR, Sinnaeve J, Ihrie RA*, Irish JM*. <a href=\"https:\/\/scholar.google.com\/citations?view_op=view_citation&amp;hl=en&amp;user=-IJndUMAAAAJ&amp;sortby=pubdate&amp;citation_for_view=-IJndUMAAAAJ:h9G0ZmjYpDoC\">Preparing Viable Single Cells from Human Tissue and Tumors for Cytomic Analysis<\/a>. <strong>Current Protocols in Molecular Biology<\/strong>\u00a02017\u00a0Apr 3;118:25C.1.1-25C.1.23. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5518778\/\">PMC5518778<\/a>. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28369679\">Pubmed<\/a>. <a href=\"https:\/\/doi.org\/10.1002\/cpmb.37\">DOI<\/a>.<\/p>\n<p style=\"text-align: justify\">\u25ba This peer-reviewed protocol for isolation and mass cytometry analysis of single cells from human tissues and tumors was developed with <a href=\"https:\/\/my.vanderbilt.edu\/ihrielab\/\">Rebecca Ihrie\u2019s lab<\/a> and is based on Leelatian and Doxie et al., <em>Cytometry B<\/em> 2017\u00a0<a href=\"http:\/\/my.vanderbilt.edu\/irishlab\/2016\/10\/published-leelatian-and-doxie-et-al-cytometry-b-2017\/\">published October 2016<\/a>\u00a0entitled\u00a0<a href=\"https:\/\/scholar.google.com\/citations?view_op=view_citation&amp;hl=en&amp;user=-IJndUMAAAAJ&amp;sortby=pubdate&amp;citation_for_view=-IJndUMAAAAJ:oYwriLWYh5YC\">Single cell analysis of human tissues and solid tumors with mass cytometry<\/a>. \u00a0Since publication, this protocol has been validated for most human and mouse tissues.\u00a0 The protocol describes tumor and tissue disaggregation into single cells and is compatible with single cell approaches like mass cytometry, cell sorting, and single cell RNA-seq.<\/p>\n<figure id=\"attachment_1067\" aria-describedby=\"caption-attachment-1067\" style=\"width: 1502px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-1067\" src=\"http:\/\/localhost\/irishv01\/wp-content\/uploads\/2017\/04\/2017-Leelatian-CPMB-Fig-1.jpg\" alt=\"Leelatian et al., Current Protocols in Molecular Biology 2017 Fig 1\" width=\"1502\" height=\"770\" srcset=\"https:\/\/www.irishlab.org\/wp-content\/uploads\/2017\/04\/2017-Leelatian-CPMB-Fig-1.jpg 1502w, https:\/\/www.irishlab.org\/wp-content\/uploads\/2017\/04\/2017-Leelatian-CPMB-Fig-1-300x154.jpg 300w, https:\/\/www.irishlab.org\/wp-content\/uploads\/2017\/04\/2017-Leelatian-CPMB-Fig-1-1024x525.jpg 1024w, https:\/\/www.irishlab.org\/wp-content\/uploads\/2017\/04\/2017-Leelatian-CPMB-Fig-1-768x394.jpg 768w\" sizes=\"auto, (max-width: 1502px) 100vw, 1502px\" \/><figcaption id=\"caption-attachment-1067\" class=\"wp-caption-text\">Leelatian et al., Current Protocols in Molecular Biology 2017 Fig 1<\/figcaption><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>Congratulations to Nalin Leelatian and co-authors on this publication! Leelatian N, Doxie DB, Greenplate AR, Sinnaeve J, Ihrie RA*, Irish JM*. Preparing Viable Single Cells from Human Tissue and Tumors for Cytomic Analysis. Current Protocols in Molecular Biology\u00a02017\u00a0Apr 3;118:25C.1.1-25C.1.23. PMC5518778. Pubmed. DOI. \u25ba This peer-reviewed protocol for isolation and mass cytometry analysis of single cells [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[],"class_list":["post-1026","post","type-post","status-publish","format-standard","hentry","category-news"],"_links":{"self":[{"href":"https:\/\/www.irishlab.org\/index.php\/wp-json\/wp\/v2\/posts\/1026","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.irishlab.org\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.irishlab.org\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.irishlab.org\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.irishlab.org\/index.php\/wp-json\/wp\/v2\/comments?post=1026"}],"version-history":[{"count":0,"href":"https:\/\/www.irishlab.org\/index.php\/wp-json\/wp\/v2\/posts\/1026\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.irishlab.org\/index.php\/wp-json\/wp\/v2\/media?parent=1026"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.irishlab.org\/index.php\/wp-json\/wp\/v2\/categories?post=1026"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.irishlab.org\/index.php\/wp-json\/wp\/v2\/tags?post=1026"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}