Irish lab on Pubmed: primary research | all research)
Recent primary research from the Irish lab:
- by Todd BartkowiakRadiographic contact of glioblastoma (GBM) tumors with the lateral ventricle and adjacent stem cell niche correlates with poor patient prognosis, but the cellular basis of this difference is unclear. Here, we reveal and functionally characterize distinct immune microenvironments that predominate […]
- by Stephanie MedinaSuspension and imaging cytometry techniques that simultaneously measure hundreds of cellular features are powering a new era of cell biology and transforming our understanding of human tissues and tumors. However, a central challenge remains in learning the identities of unexpected […]
- by Saara KavianyPatients with STAT1 gain-of-function (GOF) pathogenic variants have enhanced or prolonged STAT1 phosphorylation following cytokine stimulation and exhibit increased yet heterogeneous susceptibility to infections, autoimmunity, and cancer. Although disease phenotypes are diverse and other genetic factors contribute, how STAT1 GOF […]
- by Kevin J KramerRNA-based vaccines against SARS-CoV-2 have proven critical to limiting COVID-19 disease severity and spread. Cellular mechanisms driving antigen-specific responses to these vaccines, however, remain uncertain. Here we identify and characterize antigen-specific cells and antibody responses to the RNA vaccine BNT162b2 […]
- by Sierra M BaroneFor an emerging disease like COVID-19, systems immunology tools may quickly identify and quantitatively characterize cells associated with disease progression or clinical response. With repeated sampling, immune monitoring creates a real-time portrait of the cells reacting to a novel virus […]
- by Benjamin J ReismanFluorescent cell barcoding (FCB) enables efficient collection of tens to hundreds of flow cytometry samples by covalently marking cells with varying concentration of spectrally distinct dyes. A key consideration in FCB is to balance the density of dye barcodes, the […]
- by Sierra M BaroneFor an emerging disease like COVID-19, systems immunology tools may quickly identify and quantitatively characterize cells associated with disease progression or clinical response. With repeated sampling, immune monitoring creates a real-time portrait of the cells reacting to a novel virus […]
- by Nalin LeelatianA goal of cancer research is to reveal cell subsets linked to continuous clinical outcomes to generate new therapeutic and biomarker hypotheses. We introduce a machine learning algorithm, Risk Assessment Population IDentification (RAPID), that is unsupervised and automated, identifies phenotypically […]
- by Caroline E RoeThis article presents a single experiment designed to introduce a trainee to multiple advanced bench and analysis techniques, including high-dimensional cytometry, profiling cell signaling networks, functional assays with primary human tissue, and single-cell analysis with machine learning tools. The trainee […]
- by Mikael RousselTumor-associated macrophage and T-cell subsets are implicated in the pathogenesis of diffuse large B-cell lymphoma, follicular lymphoma, and classical Hodgkin lymphoma. Macrophages provide essential mechanisms of tumor immune evasion through checkpoint ligand expression and secretion of suppressive cytokines. However, normal […]
- by Mikael RousselThe immune monocyte/phagocyte system (MPS) includes numerous cell subsets of the myeloid lineage including monocyte, macrophage, and dendritic cell (DC) populations that are heterogeneous both phenotypically and functionally. Previously, we characterized these diverse MPS phenotypes with multi-parametric mass cytometry (CyTOF). […]
- by Kanutte HuseCD40 expression is required for germinal center (GC) formation and function, but the kinetics and magnitude of signaling following CD40 engagement remain poorly characterized in human B cells undergoing GC reactions. Here, differences in CD40 expression and signaling responses were […]
- by Akshitkumar M MistrySingle-cell technologies that can quantify features of individual cells within a tumor are critical for treatment strategies aiming to target cancer cells while sparing or activating beneficial cells. Given that key players in protein networks are often the primary targets […]
- by Allison R GreenplateAdvances in single-cell biology have enabled measurements of >40 protein features on millions of immune cells within clinical samples. However, the data analysis steps following cell population identification are susceptible to bias, time-consuming, and challenging to compare across studies. Here, […]
(all primary research on Pubmed)
Recent collaborative research from the Irish lab:
- by Maria-Fernanda Senosain
- by Allison E Norlander
- by Matthew R Alexander
- by Asa A Brockman
- by Kanutte Huse
- by Joseph A Balsamo
- by Erin M Wilfong
- by Simon Le Gallou
- by Aïda Meghraoui-Kheddar
- by Haley E Ramsey
- by Maria-Fernanda Senosain
- by Albert C Yeh
- by Lauren S Levine
- by Juliette Ferrant
- by Diana C Contreras Healey
(all research on Pubmed)
Jonathan M. Irish’s primary research from PhD & postdoc:
- by Jonathan M IrishHuman tumors contain populations of both cancerous and host immune cells whose malignant signaling interactions may define each patient's disease trajectory. We used multiplexed phospho-flow cytometry to profile single cells within human follicular lymphoma tumors and discovered a subpopulation of […]
- by Jonathan M IrishLoss or mutation of the TP53 tumor suppressor gene is not commonly observed in acute myeloid leukemia (AML), suggesting that there is an alternate route for cell transformation. We investigated the hypothesis that previously observed Bcl-2 family member overexpression suppresses […]
- by Jonathan M IrishDifferences in BCR signaling may govern outcomes as diverse as proliferation and cell death. We profiled BCR signaling kinetics in subsets of primary human B cells using flow cytometry. In the predominant population expressing IgM, BCR cross-linking led to a […]
- by Jonathan M IrishThe B-cell receptor (BCR) transmits life and death signals throughout B-cell development, and altered BCR signaling may be required for survival of B-lymphoma cells. We used single-cell signaling profiles to compare follicular lymphoma (FL) B cells and nonmalignant host B […]
- by Jonathan M IrishOncogenesis and tumour progression are supported by alterations in cell signalling. Using flow cytometry, it is now possible to track and analyse signalling events in individual cancer cells. Data from this type of analysis can be used to create a […]
- by Jonathan M IrishAltered growth factor responses in phospho-protein-driven signaling networks are crucial to cancer cell survival and pathology. Profiles of cancer cell signaling networks might therefore identify mechanisms by which such cells interpret environmental cues for continued growth. Using multiparameter flow cytometry, […]
(all primary research on Pubmed)
Jonathan M. Irish’s collaborative research from PhD & postdoc:
- by Sarah E Josefsson
- by June H Myklebust
- by Michael R Green
- by Saumyadipta Pyne
- by Michael R Green
- by June H Myklebust
- by Karen Sachs
- by Roch Houot
- by Mark M Hammer
- by Nikesh Kotecha
- by Peter O Krutzik
- by J S Armstrong
(all research on Pubmed)
Google Scholar Profile for Jonathan M. Irish:
https://scholar.google.com/citations?user=-IJndUMAAAAJ&hl=en
ORCID for Jonathan M. Irish:
https://orcid.org/0000-0001-9428-8866
NIH/Pubmed URL for Jonathan M. Irish:
http://www.ncbi.nlm.nih.gov/sites/myncbi/jonathan.irish.1/bibliography/44179499/public